Related Experiment Video
Updated: Mar 2, 2026
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (MLST)
Andrés García-Alvarez1, Ana Isabel Vela1, Elvira San Martín2
1Animal Health Department, Veterinary School, Universidad Complutense de Madrid, Spain.
Abstract:
Pasteurella multocida is a pathogen causing disease in a wide range of hosts including sheep and pigs. Isolates from ovine pneumonia were characterized by MLST (Multi-host and RIRDC databases) and virulence-associated gene (VAG) typing and compared with porcine isolates. Ovine and porcine isolates did not share any STs as determined by both schemes and exhibited different VAG profiles. With the Multi-host database, sixteen STs were identified among 43 sheep isolates with two STs (ST50 and ST19) comprising 53.5% of the isolates, and seven MLST genotypes (ST3, ST11 and ST62 included 75% of the isolates) among the 48 pig isolates. The most frequent VAG profile among sheep isolates was tbpA+/toxA+ (69.8% of isolates) and pfhA+ (62.5%) and hgbB+ (33.3%) among pig isolates. Representative ovine and porcine isolates of those STs identified by the Multi-host scheme were further typed using the RIRDC scheme. Seven STs were identified among the ovine isolates (ST95RIRDC, ST131RIRDC, ST203RIRDC, ST320RIRDC, ST324RIRDC, ST321RIRDC, and ST323RIRDC), with the latter four sequence types being new STs identified in this study, and six STs (ST9RIRDC, ST13RIRDC, ST27RIRDC, ST50RIRDC, and ST74RIRDC and a new sequence type ST322RIRDC) among the porcine isolates. STs identified among ovine isolates have been detected exclusively in small ruminants, suggesting an adaptation to these hosts, while the genotypes identified among pig isolates have been previously identified in multiple hosts and therefore they are not restricted to pigs. The differences in genotypes and VAG profiles between ovine and pig isolates suggest they could represent different subpopulations of P. multocida.
Insights
This study differentiated Pasteurella multocida strains from sheep and pigs using Multi-host and RIRDC MLST databases and VAG typing. Ovine strains showed host adaptation, while porcine strains were more widespread, suggesting distinct subpopulations.
Area of Science:
- Veterinary Microbiology
- Bacterial Pathogenesis
- Molecular Epidemiology
Background:
- Pasteurella multocida is a significant pathogen affecting various animal species, including sheep and pigs.
- Understanding genotypic and virulence factor variations is crucial for controlling P. multocida infections in different hosts.
Purpose of the Study:
- To characterize Pasteurella multocida isolates from ovine pneumonia and compare them with porcine isolates.
- To investigate the genetic diversity and virulence-associated gene profiles of P. multocida in sheep and pigs.
Main Methods:
- Multi-Locus Sequence Typing (MLST) using both Multi-host and RIRDC databases.
- Virulence-Associated Gene (VAG) typing.
- Comparative analysis of sequence types (STs) and VAG profiles between ovine and porcine isolates.
Main Results:
- Ovine and porcine P. multocida isolates did not share any sequence types (STs) and exhibited distinct VAG profiles.
- MLST analysis identified specific STs in sheep isolates (e.g., ST50, ST19) and pigs (e.g., ST3, ST11, ST62) using the Multi-host database.
- Ovine STs were predominantly found in small ruminants, indicating host adaptation, whereas porcine STs were identified in multiple hosts.
Conclusions:
- Distinct genetic subpopulations of Pasteurella multocida exist in sheep and pigs.
- Differences in MLST genotypes and VAG profiles suggest host-specific adaptations and distinct evolutionary trajectories for ovine and porcine P. multocida strains.
More Related Videos
10:33Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019
11:32Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
Published on: February 23, 2014
