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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
Published on: May 29, 2012
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Applications of Raman Spectroscopy in Biopharmaceutical Manufacturing: A Short Review
1Nanoscale Biophotonics Laboratory, School of Chemistry, National University of Ireland - Galway, Galway, Ireland.
Applied Spectroscopy
|May 24, 2017
Summary
Raman spectroscopy offers unique analytical solutions for biopharmaceutical manufacturing, enabling online process monitoring. However, its application is limited by fluorescence and signal strength, requiring careful experimental design for robust results.
Area of Science:
- Biopharmaceutical Manufacturing
- Analytical Chemistry
- Process Analytical Technology (PAT)
Background:
- The biopharmaceutical industry is rapidly expanding, driven by complex macromolecule-based drugs (biologics).
- Current manufacturing processes for biologics face significant analytical challenges due to their complexity and cell-based production methods.
- Existing Process Analytical Technology (PAT) in small molecule manufacturing is advanced, but biologic manufacturing lags behind in analytical control.
Purpose of the Study:
- To review and discuss the applications of Raman spectroscopy in biopharmaceutical manufacturing, particularly in mammalian cell culture.
- To highlight the potential of Raman spectroscopy as a Process Analytical Technology (PAT) tool for biologics.
- To identify the limitations and future potential of Raman spectroscopy in this field.
Main Methods:
- Review of existing literature on Raman spectroscopy applications in biopharmaceutical process monitoring.
- Emphasis on mammalian cell culture as a representative biological expression system.
- Discussion of Raman spectroscopy's properties, advantages, and limitations in the context of bioprocessing.
Main Results:
- Raman spectroscopy demonstrates significant advantages for biopharmaceutical online process monitoring, including non-destructive, non-contact measurement and minimal sample preparation.
- Successful applications have been identified, providing unique analytical solutions for process control.
- Limitations such as fluorescence interference and weak signal intensity, especially in complex cell culture media, restrict quantitative analysis.
Conclusions:
- Raman spectroscopy offers valuable tools for biopharmaceutical process monitoring, but its limitations necessitate careful experimental design and awareness of potential interferences.
- New technological advancements like time-resolved detectors and plasmonics may help overcome current limitations and offer new analytical insights.
- Further development is needed to fully realize the potential of Raman spectroscopy for robust quantitative analysis in bioprocessing.
Keywords:
Raman spectroscopybiopharmaceutical manufacturingcell culture mediachemometricsonline monitoringproteinsMore Related Videos
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