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Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
Marcelo Henrique Santos Paiva1,2, Duschinka Ribeiro Duarte Guedes1, Walter Soares Leal3
1Centro Acadêmico do Agreste, Universidade Federal de Pernambuco, Caruaru, PE, Brazil.
Genetics and Molecular Biology
|May 24, 2017
Abstract:
Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).

