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Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
Published on: June 13, 2019
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HIF-1α inhibits IDH-1 expression in osteosarcoma
Deng-Cheng Liu1, Xun Zheng1, Yong Zho1
1Zhongnan Hospital of Wuhan University, Department of Orthopedics, Wuhan University, Wuhan, Hubei 430071, P.R. China.
Oncology Reports
|May 24, 2017
Summary
Hypoxia inducible factor-1 (HIF-1) inhibits isocitrate dehydrogenase 1 (IDH-1) in osteosarcoma. This inhibition by HIF-1 may contribute to the increased incidence of osteosarcoma, highlighting a novel therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Hypoxia inducible factor-1 (HIF-1) and isocitrate dehydrogenase 1 (IDH-1) are implicated in various cancers.
- The roles of HIF-1 and IDH-1 in osteosarcoma remain largely unexplored.
Purpose of the Study:
- To investigate the expression and correlation of HIF-1 and IDH-1 in osteosarcoma.
- To elucidate the functional relationship between HIF-1 and IDH-1 in osteosarcoma cells.
Main Methods:
- Immunohistochemistry on 35 osteosarcoma tissue samples.
- Western blotting in MG-63 and 143B osteosarcoma cell lines under normoxic and hypoxic conditions.
- Lentiviral vector-mediated RNA interference to downregulate HIF-1α.
Main Results:
- A negative correlation was observed between HIF-1α and IDH-1 expression in osteosarcoma tissues.
- Hypoxia increased HIF-1α expression while decreasing IDH-1 expression in osteosarcoma cell lines.
- Downregulation of HIF-1α led to increased IDH-1 expression in MG-63 cells.
Conclusions:
- HIF-1α acts as an inhibitor of IDH-1 in osteosarcoma.
- The HIF-1α/IDH-1 pathway may play a significant role in osteosarcoma development and progression.
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