Identification of Forensically Important Blow Flies (Diptera: Calliphoridae) in China Based on COI

Blow flies are among the most important insects in forensic entomology casework. Identification of blow fly species can be a time consuming and difficult task, especially at their early development stages. Present DNA-based technologies provide a promising identification method for these forensically important calliphorids. The cytochrome oxidase subunit I (COI) sequence has been applied as a suitable DNA marker in calliphorid identification for many years; however, limitation exists in using short sequence to determine genetically close species. In this study, COI long sequences were utilized in species-level identification. Seventy-two specimens were collected from 27 locations across 22 Chinese provinces, and unambiguously determined as 16 species under seven genera of Calliphoridae. Analysis of long mitochondrial COI sequence (1,021-1,382 bp) data from forensically relevant blow flies collected in the inland region of China provided a reliable marker for accurate identification. Our data provide genetic diversity and reference for global forensic-related blow fly species identification, and conductive meaning on future utilization of Chinese calliphorids used in forensic entomological practice.