Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new method of sequencing DNA.

E D Hyman1

  • 1Sybtrel Biotechnology, Columbia, South Carolina 29223.

Analytical Biochemistry
|November 1, 1988
PubMed
Summary
This summary is machine-generated.

A novel DNA sequencing method bypasses electrophoresis, radioactivity, and fluorescence. It measures pyrophosphate produced during DNA synthesis, enabling continuous monitoring for accurate sequencing. This innovation promises automated DNA analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation of plasmid DNA by sequential enzymatic digestion.

BioTechniques·1992
See all related articles

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Traditional DNA sequencing methods often rely on electrophoresis, radioactivity, or fluorescence, which can be complex and costly.
  • There is a need for simpler, more accessible DNA sequencing technologies.

Purpose of the Study:

  • To develop and demonstrate a novel DNA sequencing method.
  • To establish a technique that avoids the use of electrophoresis, radioactivity, and fluorescence.

Main Methods:

  • The new method measures pyrophosphate generated during DNA polymerization.
  • DNA and DNA polymerase are immobilized on a DEAE-Sepharose column.
  • Sequential solutions of different deoxynucleotide triphosphates (dNTPs) are introduced.
  • Pyrophosphate is continuously detected using an enzyme-based detection system on Sepharose columns.

Related Experiment Videos

Main Results:

  • The method successfully sequenced an alternating copolymer poly(dA.dT).
  • Continuous pyrophosphate measurement allows for real-time monitoring of DNA synthesis.

Conclusions:

  • A new, non-electrophoretic, non-radioactive, and non-fluorescent DNA sequencing method has been successfully developed.
  • The pyrophosphate detection system offers a viable alternative for DNA sequencing.
  • Future improvements can facilitate the automation of this sequencing technique.