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The Deubiquitinating Enzymes UBP12 and UBP13 Positively Regulate MYC2 Levels in Jasmonate Responses

Jin Seo Jeong1, Choonkyun Jung1, Jun Sung Seo1

  • 1Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065.

The Plant Cell
|May 25, 2017
PubMed

Insights

Deubiquitinating enzymes UBP12 and UBP13 stabilize the MYC2 protein, a key regulator of jasmonate (JA) responses. This stabilization enhances plant sensitivity to JA, revealing a new regulatory mechanism in plant signaling.

Area of Science:

  • Plant Molecular Biology
  • Plant Hormone Signaling
  • Ubiquitin-Proteasome System

Background:

  • The transcription factor MYC2 is a master regulator of jasmonate (JA)-mediated responses and signaling pathway crosstalk.
  • MYC2 protein stability is regulated by the PUB10 E3 ligase, which mediates its polyubiquitination and subsequent degradation.

Purpose of the Study:

  • To investigate the role of deubiquitinating enzymes UBP12 and UBP13 in regulating MYC2 stability and JA responses.
  • To determine if UBP12 and UBP13 can counteract the destabilizing effect of PUB10 on MYC2.

Main Methods:

  • In vitro deubiquitination assays using polyubiquitinated MYC2 and UBP12/UBP13.
  • Co-immunoprecipitation to assess the association of UBP12/UBP13 with MYC2 in the nucleus.
  • Analysis of MYC2 protein levels and JA sensitivity in transgenic Arabidopsis thaliana plants with altered UBP12/UBP13 expression.

Main Results:

  • UBP12 and UBP13 were shown to deubiquitinate polyubiquitinated MYC2 in vitro.
  • UBP12 and UBP13 were found to associate with MYC2 in the nucleus.
  • Arabidopsis plants lacking UBP12/UBP13 exhibited faster MYC2 degradation and reduced JA sensitivity.
  • Conversely, plants overexpressing UBP12/UBP13 showed increased MYC2 stability and enhanced JA sensitivity.

Conclusions:

  • UBP12 and UBP13 act as positive regulators of JA responses by stabilizing the MYC2 transcription factor.
  • These deubiquitinating enzymes counteract the action of the PUB10 E3 ligase, providing a novel mechanism for MYC2 regulation.
  • A genetic link between UBP12, UBP13, and MYC2 in JA signaling pathways was established.

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