Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammation01:38

Inflammation

62.9K
Overview
62.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Post-recanalization administration of rapamycin improves functional outcome and reduces infarct size in a rat model of ischemic stroke.

Neuroprotection (Chichester, England)·2026
Same author

Adipose-Cardiac Crosstalk in Obesity-Related Atrial Fibrillation: The Emerging Role of Extracellular Vesicles.

JACC. Basic to translational science·2026
Same author

Targeted Intracellular Delivery of Amino Acids to Trophoblast Cells Reveals Proteomic Signatures of Cellular Utilisation.

Biomolecules·2026
Same author

Feasibility of multimodal metabolic analysis for detecting early changes in acute neuroinflammation.

Journal of neuroinflammation·2026
Same author

Therapeutic TG2 inhibition reverses systemic multiomic dysregulation in celiac disease.

BMC medicine·2026
Same author

Prenatal and Postnatal Maternal Psychological Distress and the Metabolomic Profile of Human Milk.

The Journal of nutrition·2026

Related Experiment Video

Updated: Mar 2, 2026

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
10:09

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

7.4K

Inflammatory Stroke Extracellular Vesicles Induce Macrophage Activation.

Yvonne Couch1, Naveed Akbar2, Simon Davis2

  • 1From the Acute Stroke Programme, Radcliffe Department of Medicine, John Radcliffe Hospital (Y.C., A.A.N., A.M.B.), Division of Cardiovascular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital (N.A.), Target Discovery Institute, Nuffield Department of Medicine (S.D., R.F.), and Centre for Prevention of Stroke and Dementia, John Radcliffe Hospital, Nuffield Department of Clinical Neurosciences (P.M.R., A.I.B.), University of Oxford, United Kingdom; and Turku Centre for Biotechnology, University of Turku, Biocity, Finland (A.M.D.). yvonne.couch@rdm.ox.ac.uk.

Stroke
|May 25, 2017
PubMed
Summary

Extracellular vesicles (EVs) from stroke patients are more numerous and contain proteins like C-reactive protein. These stroke EVs can trigger inflammation in immune cells, highlighting their proinflammatory nature.

Keywords:
C-reactive proteinacute-phase proteinsextracellular vesiclesmacrophagesstroke

More Related Videos

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
13:28

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Published on: September 4, 2013

12.0K
Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
12:14

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Published on: February 12, 2016

36.3K

Related Experiment Videos

Last Updated: Mar 2, 2026

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
10:09

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

7.4K
Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
13:28

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Published on: September 4, 2013

12.0K
Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
12:14

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Published on: February 12, 2016

36.3K

Area of Science:

  • Neuroscience
  • Immunology
  • Biochemistry

Background:

  • Extracellular vesicles (EVs) are cell-released complexes involved in physiological and pathological processes.
  • Stroke is a pathological condition associated with cellular dysfunction and inflammation.

Purpose of the Study:

  • To investigate the inflammatory potential of EVs isolated from stroke patients.
  • To characterize the proteomic profile of stroke-associated EVs.

Main Methods:

  • Quantification and proteomic analysis (LC-MS/MS) of EVs in acute stroke patient sera.
  • Application of isolated EV fractions to macrophage cultures to assess inflammatory gene expression.

Main Results:

  • Significantly increased EV number, but not size, in stroke patients compared to controls.
  • Proteomic analysis revealed elevated acute phase proteins, including C-reactive protein, in stroke EVs.
  • Stroke-derived EVs induced inflammatory gene expression in macrophages.

Conclusions:

  • EVs from stroke patients exhibit a proinflammatory phenotype.
  • Stroke EVs are capable of inducing inflammation in immune cells, suggesting a role in stroke-related inflammatory responses.