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Updated: Mar 2, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
Published on: February 23, 2021
Natural antisense RNAs as mRNA regulatory elements in bacteria: a review on function and applications
Fatemeh Saberi1, Mehdi Kamali2, Ali Najafi1
1Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Abstract:
Naturally occurring antisense RNAs are small, diffusible, untranslated transcripts that pair to target RNAs at specific regions of complementarity to control their biological function by regulating gene expression at the post-transcriptional level. This review focuses on known cases of antisense RNA control in prokaryotes and provides an overview of some natural RNA-based mechanisms that bacteria use to modulate gene expression, such as mRNA sensors, riboswitches and antisense RNAs. We also highlight recent advances in RNA-based technology. The review shows that studies on both natural and synthetic systems are reciprocally beneficial.
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