A target triggered proximity combination-based fluorescence sensing strategy for adenosine detection

Area of Science:

• Biochemistry
• Analytical Chemistry
• Molecular Diagnostics

Background:

• Adenosine is a critical physiological regulator with abnormal levels linked to various diseases.
• Sensitive and specific detection of adenosine is essential for health monitoring and disease diagnosis.

Purpose of the Study:

• To develop a target-triggered proximity combination-based fluorescence sensing strategy for adenosine detection.
• To design a difunctional probe for enhanced sensitivity and specificity in adenosine sensing.

Main Methods:

• A sensing strategy integrating split aptamers, strand displacement amplification (SDA), and rolling circle amplification (RCA).
• Utilized a fragment-elongated polymerase/nicking template and Thioflavin T for signal amplification.
• Designed a difunctional probe for adenosine recognition and signal generation.

Main Results:

• Achieved a linear correlation for adenosine detection from 5.0 × 10⁻⁷ M to 2.0 × 10⁻⁵ M (R = 0.999).
• Demonstrated a low detection limit of 8.4 × 10⁻⁸ M with high selectivity against adenosine analogues.
• Showed successful application in human serum samples with recoveries ranging from 91% to 94%.

Conclusions:

• The developed fluorescence sensing strategy offers sensitive and specific detection of adenosine.
• The method shows promise for applications in biochemical research, clinical diagnosis, and disease treatment.
• The proximity-based approach with DNA amplification provides a robust platform for biomarker detection.