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Studies on nuclear acid deoxyribonucleases and proofreading exonuclease isolated from rat liver nuclei
1Department of Health Sciences, Faculty of Integrated Arts and Sciences, University of Tokushima.
Journal of Biochemistry
|October 1, 1988
Abstract:
Three kinds of deoxyribonucleases (peaks A, B, and C) were separated from purified rat liver nuclei on DEAE-cellulose column chromatography, and their characteristics were partially studied. Peaks A and B had endonuclease activities under acidic conditions with low substrate specificity and did not require divalent metal ions. Peak C had an exonuclease activity under alkaline conditions with substrate specificity for denatured DNA or single stranded homopolymer and required divalent cations. Peak C degraded 3'-terminally mismatched substrate much faster than 3'-terminally matched substrate.