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Functionally active thrombomodulin is present in human platelets.

K Suzuki1, J Nishioka, T Hayashi

  • 1Department of Laboratory Medicine, Mie University School of Medicine.

Journal of Biochemistry
|October 1, 1988
PubMed
Summary

Human platelets contain active thrombomodulin, which accelerates protein C activation by thrombin. This platelet cofactor activity is similar to placental thrombomodulin, suggesting a role in protein C regulation on platelet surfaces.

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Area of Science:

  • Biochemistry
  • Hematology
  • Cell Biology

Background:

  • Thrombomodulin is a key regulator of the protein C anticoagulant pathway.
  • Its presence and function in human platelets have not been fully elucidated.

Purpose of the Study:

  • To investigate the presence and functional activity of thrombomodulin in human platelets.
  • To compare platelet thrombomodulin with placental thrombomodulin.

Main Methods:

  • Quantification of thrombomodulin in human platelets.
  • Assay of protein C activation by thrombin with and without platelets.
  • Partial purification of platelet thrombomodulin using affinity chromatography.
  • Biochemical characterization of purified platelet thrombomodulin (molecular weight, cofactor activity, binding kinetics).

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Main Results:

  • Functionally active thrombomodulin was detected in human platelets (60 +/- 18 molecules/platelet).
  • Platelets significantly accelerated thrombin-mediated protein C activation, an activity neutralized by anti-thrombomodulin antibodies.
  • Purified platelet thrombomodulin exhibited biochemical properties (Mr, affinity for thrombin and protein C) identical to placental thrombomodulin.

Conclusions:

  • Human platelets express functional thrombomodulin.
  • Platelet thrombomodulin acts as a cofactor for protein C activation by thrombin.
  • Thrombomodulin on platelet surfaces may play a significant role in regulating the protein C pathway in vivo.