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Updated: Mar 1, 2026
In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury
Published on: June 8, 2013
The effect of intradermal administration of inactive platelet-rich plasma on flap viability in rats
Erkan Orhan1, Ahmet Çağrı Uysal2, Engin Başer3
1Assistant Professor, Department of Plastic Surgery, Namik Kemal University, Tekirdağ, Turkey. Scientific, intellectual, conception and design of the study; acquisition, analysis and interpretation of data; technical procedures; statistical analysis; manuscript preparation and writing; final approval.
Purpose::
To evaluate the effect of inactive form of platelet rich plasma (PRP) on the flap viability.
Methods::
Thirty six rats were used. Rats were divided into six groups then 9x3 cm random pattern skin flaps were elevated from dorsum of all rats. For precluding vascularization from the base, a silicone layer was placed under the flap in groups 2(only flap+silicone), 4(saline+silicone) and 6(PRP+silicone). In groups 1(only flap), 2(only flap+silicone) nothing was done except flap surgery. In groups 3(saline) and 4(saline+silicone), saline was applied intradermally , in groups 5(PRP) and 6(PRP+silicone), inactive form of PRP which obtained from different 16 rats was applied intradermally, into certain points of flaps immediately after surgery. After 7 days flap necrosis ratio was measured in all groups.
Results::
Mean necrosis rate in group 5(PRP) (16.05%) was statistically significantly lower than group 1(only flap) (31,93%) and group 3(saline) (30,43%) (p<0.001). Mean necrosis rate in group 6(PRP+silicone) (36.37%) was statistically significantly lower than group 2(only flap+silicone) (47.93%) and group 4(saline+silicone) (45.65%) (p<0.001).
Conclusion::
Intradermal inactive platelet rich plasma administration decreases flap necrosis so for skin application.
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