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Updated: Mar 1, 2026
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
Published on: July 25, 2016
[Expression of FABP7 in mouse placenta tissue and human trophoblast HTR-8/Svneo cells]
Liu Tian1, Hui-Qi Liao, Hui Yang
1Reproductive Medicine Center, People's Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, China.E-mail: 738652051@qq.com.
Objective:
To detect the expression of FABP7 in the placenta of pregnant mice and in HTR-8/Svneo cells.
Methods:
Real-time PCR and immunofluorescence were used to detect FABP7 mRNA and protein expressions in the uterine and placental tissue of pregnant mice at different days of gestation. FABP7 expression was also detected in cultured HTR-8/Svneo cells using immunofluorescence assay. The mice were treated with E2, P4 or their combination for 6 and 24 h and Fabp7 mRNA level in the uterus was detected with real-time PCR.
Results:
At 7.5-10.5 days of gestation, the pregnant mice showed positive expressions of Fabp7 mRNA in the uterus and placenta, and FABP7 protein was detected in the decidualized cells and trophoblast giant cells. The expressions of FABP7 were detected at both the mRNA and protein levels in cultured HTR-8/Svneo cells. In mice treated with P4 alone or with E2+P4 for 6 and 24 h, the expression level of Fabp7 mRNA was upregulated in the uterus. Fabp7 upregulation was observed in mice at 24 h following E2 treatment but not at 6 h.
Conclusion:
FABP7 is expressed in trophoblast giant cells and decidual cells in the placental tissue of mice and in cultured HTR-8/Svneo cells, suggesting the involvement of FABP7 in placental development and in maintenance of pregnancy. E2 and P4 can regulate the expression of FABP7 in mouse uterus.
