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Plasma-activated water: antibacterial activity and artifacts?

Tung-Po Chen1, Junfeng Liang2, Tsan-Liang Su3

  • 1Department of Civil, Environmental and Ocean Engineering, Stevens Institute of Technology, Hoboken, NJ, 07307, USA.

Environmental Science and Pollution Research International
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Summary

Plasma-activated water (PAW) shows antibacterial and anti-biofilm effects due to acidification and metal ions. However, researchers found potential artifacts in reported PAW biological activities, urging careful consideration.

Keywords:
Anti-biofilm activityAntibacterial activityBiofilmMetalNon-thermal plasmaPlasma-activated water

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Area of Science:

  • Biochemistry
  • Microbiology
  • Plasma Physics

Background:

  • Plasma-activated water (PAW) exhibits broad biological activities, including antibacterial and anti-biofilm effects.
  • PAW's mechanisms involve bacterial membrane damage and diffusion into extracellular matrices.
  • Trace metal ions and solution acidification are implicated in PAW's biological effects.

Purpose of the Study:

  • To investigate the key factors contributing to the antibacterial activity of PAW.
  • To determine the role of metal ions and solution acidification in PAW's biological effects.
  • To identify potential artifacts in reported PAW biological activities.

Main Methods:

  • Treatment of water using gas discharge plasma.
  • Analysis of PAW composition, including trace metal ions (copper, zinc).
  • Assessment of antibacterial and anti-biofilm activities.
  • Evaluation of solution acidification effects.

Main Results:

  • PAW demonstrated significant antibacterial and anti-biofilm activity.
  • Trace amounts of copper and zinc ions were found to play a key role in bacterial inactivation.
  • Solution acidification caused by reactive species was essential for the observed activities.
  • The contribution of metal ions varied depending on the plasma working gases used.

Conclusions:

  • While PAW shows promising antibacterial and anti-biofilm properties, metal ions and acidification are crucial contributing factors.
  • Potential artifacts in reported biological activities of PAW should be carefully considered in future research.
  • Further studies are needed to fully elucidate the complex mechanisms underlying PAW's biological effects.