Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

239.0K
Overview
239.0K
Next-generation Sequencing03:00

Next-generation Sequencing

99.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
99.6K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

7.4K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.4K
Real Time RT-PCR02:57

Real Time RT-PCR

65.8K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.8K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

62.0K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
62.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A primary care pharmacogenetic precision medicine pilot based on specific Māori tribal ethical frameworks and principles.

Journal of community genetics·2026
Same author

Rho-associated kinase (ROCK)-associated proteins and genes-encoded proteins upregulated in lung squamous cell carcinoma (LUSC).

Journal of the Egyptian National Cancer Institute·2026
Same author

The extracellular matrix: structure, composition, biological functions, diseases, and therapeutic targets.

Molecular biomedicine·2026
Same author

Exploring the perspective of Malaysian Community Pharmacists on Sports Pharmacy Services: A qualitative study.

Journal of public health research·2026
Same author

Allelic diversity of the pharmacogenes CYP2D6 and CYP2C19 in Māori from Te Tairāwhiti, Aotearoa New Zealand.

Frontiers in genetics·2026
Same author

Assessment of Maladaptive Exercise History Among Individuals With Eating Disorders: Validation of a Brief, Self-Report Measure.

European eating disorders review : the journal of the Eating Disorders Association·2026

Related Experiment Video

Updated: Mar 1, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

30.9K

Long Fragment Polymerase Chain Reaction.

Eng Wee Chua1, Simran Maggo2, Martin A Kennedy2

  • 1Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, 50300, Malaysia. cew85911@ukm.edu.my.

Methods in Molecular Biology (Clifton, N.J.)
|May 26, 2017
PubMed
Summary

Long-range PCR amplifies large DNA fragments, overcoming limitations of standard PCR. This protocol successfully generated large DNA products up to 20 kb from human samples.

Keywords:
Agarose gel electrophoresisLong ampliconsLong-range polymerase chain reaction (PCR)Proofreading enzymeTaq DNA polymeraseThermal cycling

More Related Videos

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

3.2K
Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

19.8K

Related Experiment Videos

Last Updated: Mar 1, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

30.9K
Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

3.2K
Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

19.8K

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Standard Polymerase Chain Reaction (PCR) is limited in amplifying large DNA fragments due to polymerization errors and template degradation.
  • Amplifying extensive DNA regions is crucial for various downstream genetic analyses.

Purpose of the Study:

  • To describe a protocol for Long-Range PCR (LR-PCR).
  • To demonstrate the successful amplification of large DNA fragments exceeding several kilobases (kb).

Main Methods:

  • Utilized a modified PCR technique specifically designed for amplifying long DNA sequences.
  • Applied the protocol to human genomic DNA samples.

Main Results:

  • Successfully amplified DNA fragments of 6.6 kb, 7.2 kb, 13 kb, and 20 kb.
  • Demonstrated the efficacy of LR-PCR in generating large DNA products.

Conclusions:

  • The described Long-Range PCR protocol effectively overcomes limitations of standard PCR for large DNA fragment amplification.
  • This method provides a reliable means to obtain substantial DNA amplicons from genomic DNA for further research.