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Determination of delta 9-tetrahydrocannabinol in plasma using solid-phase extraction and high-performance liquid
P G Zweipfenning1, J A Lisman, A Y van Haren
1Forensic Science Laboratory, Rijswijk, The Netherlands.
Journal of Chromatography
|December 2, 1988
Abstract:
A method for the determination of nanogram amounts of delta 9-tetrahydrocannabinol (THC) in plasma and serum is described. THC was quantitatively isolated by solid-phase extraction after addition of an aqueous solution of urea and methanol to the sample. The extracts were analysed by high-performance liquid chromatography with electrochemical detection in the oxidizing mode. The detection limit of THC is ca. 100 pg for a signal-to-noise ratio of 3. With this method, levels of 2 ng/ml of THC in plasma can be measured.