Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.2K
CRISPR01:59

CRISPR

58.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.3K
Homologous Recombination02:31

Homologous Recombination

64.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted mutagenesis and base editing using engineered <i>Brevibacillus laterosporus</i> Cas9 with expanded target scope in rice.

Plant biotechnology (Tokyo, Japan)·2026
Same author

Polθ-mediated T-DNA integration is a conserved mechanism across plant species.

The Plant journal : for cell and molecular biology·2026
Same author

Haplotype bias detection using pedigree-based transmission simulation: traces of selection that occurred in apple breeding.

Horticulture research·2026
Same author

Efficient and accurate prime editing system in plants.

The New phytologist·2026
Same author

Guidelines for Nutritional Management of Sarcopenia and Frailty 2025. Guest Editor: Masafumi Kuzuya. This publication has been supported by The Japan Geriatrics Society and National Center for Geriatrics and Gerontology (NCGG) (Japan).

Geriatrics & gerontology international·2026
Same author

Florigen and cytokinin signaling antagonistically regulate FLOWERING LOCUS T-LIKE1 to drive a florigen relay that facilitates inflorescence development in rice.

Science advances·2025

Related Experiment Video

Updated: Mar 1, 2026

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

8.2K

CRISPR/Cas9-mediated targeted mutagenesis in grape.

Ikuko Nakajima1, Yusuke Ban1, Akifumi Azuma1

  • 1Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization, Fujimoto, Tsukuba, Ibaraki, Japan.

Plos One
|May 26, 2017
PubMed
Summary

The CRISPR/Cas9 genome editing tool successfully created targeted mutations in grape plants (Vitis vinifera). This breakthrough opens doors for genetic improvement in this important fruit crop.

More Related Videos

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.3K
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.9K

Related Experiment Videos

Last Updated: Mar 1, 2026

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

8.2K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.3K
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.9K

Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • CRISPR/Cas9 genome editing is established in many plants but underutilized in grape (Vitis vinifera).
  • Grape is a significant fruit crop for both table consumption and wine production.
  • Developing genome editing tools for grape is crucial for crop improvement.

Purpose of the Study:

  • To report the first successful targeted mutagenesis in grape using the CRISPR/Cas9 system.
  • To demonstrate the efficacy of CRISPR/Cas9 for genetic modification in Vitis vinifera.

Main Methods:

  • Transformation of grape embryonic calli with Cas9 and sgRNA constructs targeting the Vitis vinifera phytoene desaturase (VvPDS) gene.
  • Regeneration of edited plants from transformed calli.
  • DNA sequencing to confirm mutations at the VvPDS target site.

Main Results:

  • Regenerated grape plants exhibited albino leaves, indicating successful VvPDS gene disruption.
  • DNA sequencing confirmed targeted mutations in the VvPDS gene in regenerated plants.
  • A higher ratio of mutated cells was observed in older leaves compared to younger leaves.

Conclusions:

  • The CRISPR/Cas9 system is effective for targeted mutagenesis in grape (Vitis vinifera).
  • The observed mutation patterns suggest potential differences in DNA repair efficiency or DSB induction in grape tissues.
  • This study provides a foundation for future genetic engineering of grape varieties.