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Engineering muscle cell alignment through 3D bioprinting.

Pamela Mozetic1, Sara Maria Giannitelli1, Manuele Gori1

  • 1Department of Engineering, Tissue Engineering Laboratory, Università Campus Bio-Medico di Roma, via Álvaro del Portillo 21, Rome, 00128, Italy.

Journal of Biomedical Materials Research. Part A
|May 26, 2017
PubMed
Summary
This summary is machine-generated.

Additive manufacturing of hydrogels using Pluronic/alginate blends enables direct fabrication of cell-laden tissue precursors. This method promotes cell alignment and enhances myogenic gene expression, overcoming limitations of traditional tissue engineering scaffolds.

Keywords:
cell alignmentcell-laden hydrogel bioprintingskeletal muscle regenerationtissue engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Additive Manufacturing

Background:

  • Hydrogel processing is a key challenge for additive manufacturing (AM) in soft tissue engineering.
  • Directly manufacturing tissue precursors with native-like cell density can reduce extensive in vitro culture.
  • Tailoring structural and functional properties of engineered tissues is crucial for therapeutic applications.

Purpose of the Study:

  • To develop a simple AM methodology for fabricating cell-laden tissue precursors.
  • To utilize the thermoresponsive behavior of block copolymers for shape retention and cell alignment.
  • To investigate Pluronic/alginate blends as a model system for myoblast cell line processing.

Main Methods:

  • Additive manufacturing (AM) methodology utilizing thermoresponsive Pluronic block copolymers.
  • Formulation of Pluronic/alginate blends for processing of C2C12 murine myoblast cell line.
  • Assessment of shape retention at physiological conditions, cell alignment, cell viability, and gene expression.

Main Results:

  • The AM methodology achieved good shape retention at physiological conditions.
  • C2C12 cells demonstrated alignment along the deposition direction within the hydrogel constructs.
  • Fabricated constructs showed high cell viability and significantly improved expression of myogenic genes compared to 2D cultures.

Conclusions:

  • The developed AM method offers a novel approach to control cell histoarchitecture during fabrication.
  • Pluronic/alginate blends are suitable for direct 3D bioprinting of myoblasts.
  • This technique holds promise for advancing soft tissue engineering by enabling direct fabrication of functional tissue constructs.