Related Experiment Video
Updated: Mar 1, 2026
A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources
Published on: January 27, 2026
Comparative study of two chemical protocols for creating white spot lesions: An in vitro FluoreCam evaluation
Moufida Abufarwa1, Amal Noureldin2, Phillip M Campbell3
1Department of Biomedical Sciences, Texas A&M University College of Dentistry, Dallas, TX, USA.
Aim:
The aim of the present study was to compare the effectiveness and practicality of two commonly-used protocols for white spot lesion creation.
Methods:
Sound posterior human teeth were sectioned into halves and randomly allocated into two groups (n=20). Using the FluoreCam system, the enamel surfaces were imaged under standardized conditions, and baseline data (area, intensity, and impact) were recorded. Specimens were covered with an acid-resistant polish, leaving a 2×6-mm enamel window. Group 1 was immersed in Queiroz solution (64 hours/37°C/pH5 with agitation); Group 2 was immersed in Buskes solution (14 days/37°C/pH5 with agitation). The specimens then were imaged again. Within-group changes that occurred over time were evaluated using the Wilcoxon signed-rank test. Between-group differences were analyzed using the Mann-Whitney U-test. Polarized light microscopy (PLM) was used to calculate the mean lesion depth of representative specimens.
Results:
Each protocol showed significant changes in the surface area, intensity and impact of demineralization over time (P<.05). There were no statistically-significant differences (P=.53, P=.2, P=.74) between the two groups. PLM showed that the mean lesion depths of representative specimens were 51.4 and 73.0 μm for groups 1 and 2, respectively.
Conclusions:
While both protocols produce similar amounts of demineralization, the Queiroz solution is more practical, as it requires less time.
