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Systems-wide Studies Uncover Commander, a Multiprotein Complex Essential to Human Development.

Anna L Mallam¹, Edward M Marcotte¹

¹Department of Molecular Biosciences, Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA.

|May 26, 2017

Summary

This summary is machine-generated.

Commander, a newly discovered multiprotein complex, is vital for fundamental cellular functions and vertebrate development. Its disruption impacts craniofacial, brain, and heart formation, suggesting roles in developmental disorders.

Keywords:

Commander complex developmental disorders endosomal protein sorting mammalian interactome multiprotein complex system-wide studies

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Area of Science:

Cell Biology
Proteomics
Developmental Biology

Background:

Mass spectrometry has revealed a large multiprotein complex, named Commander, conserved across animals and ubiquitously expressed in human cells.
Commander is hypothesized to perform fundamental cellular functions, similar to other major cellular machinery involved in gene expression, protein homeostasis, and trafficking.

Purpose of the Study:

To review the discovery, composition, structure, and function of the Commander complex.
To explore the synergy between large-scale proteomics and focused studies in identifying Commander.
To establish parallels between Commander and the retromer complex for future research directions.

Main Methods:

Utilized mass spectrometry to map the human interactome and identify Commander.
Conducted experiments on individual subunits to elucidate functional roles.
Employed 3D modeling and biochemical data to analyze complex structure and compare it to known complexes.

Main Results:

Commander is a broadly conserved, ubiquitously expressed multiprotein complex.
Subunit studies indicate roles in endosomal protein sorting, including trafficking of Notch proteins, copper transporters, and lipoprotein receptors.
Commander is essential for vertebrate embryogenesis; defects cause craniofacial, brain, and heart developmental disruptions.

Conclusions:

Commander plays a fundamental role in cellular processes and vertebrate development.
The discovery of Commander highlights the power of systems biology approaches.
Commander shares structural and functional similarities with the retromer complex, providing a basis for investigating its role in human developmental disorders.