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Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
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Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
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Fibronectin-based multilayer thin films.

Adeline Gand1, Maud Tabuteau1, Coline Chat1

  • 1Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (ERRMECe), Institut des Matériaux, Université de Cergy-Pontoise, 95302 Cergy-Pontoise Cedex, France.

Colloids and Surfaces. B, Biointerfaces
|May 26, 2017
PubMed
Summary
This summary is machine-generated.

Researchers created nanoscale films mimicking the extracellular matrix (ECM) using poly(l-lysine) (PLL) and fibronectin (Fn). These ECM-mimicking films enhanced pre-osteoblastic cell proliferation and maintained fibronectin in a more biologically active state.

Keywords:
Cell-material interactionExtracellular matrixFibronectinLayer-by-layer assemblyMultilayer film

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Nanotechnology

Background:

  • Extracellular matrix (ECM) structure and composition are crucial for cell function.
  • Developing synthetic biomaterials that mimic the ECM is essential for regenerative medicine and tissue engineering.
  • Nanoscale films offer a platform to control biomaterial-cell interactions.

Purpose of the Study:

  • To construct nanoscale films mimicking the ECM using layer-by-layer (LbL) assembly of poly(l-lysine) (PLL) and fibronectin (Fn).
  • To investigate the growth characteristics and structural properties of these PLL-Fn films.
  • To evaluate the effect of these ECM-mimicking films on pre-osteoblastic cell behavior.

Main Methods:

  • Layer-by-layer (LbL) assembly of poly(l-lysine) (PLL) and fibronectin (Fn).
  • Characterization of film thickness, charge, and structure using spectroscopy and microscopy.
  • Assessment of murine MC3T3-E1 pre-osteoblastic cell proliferation and fibronectin state on the films.

Main Results:

  • LbL assembly of PLL-Fn films showed sub-linear growth and saturation after approximately 10 layers.
  • Formation of fibronectin aggregates was observed, correlating with diminished charge reversal and suppressed LbL assembly.
  • PLL-Fn films significantly enhanced MC3T3-E1 cell proliferation compared to a fibronectin monolayer.
  • These films maintained a higher proportion of fibronectin in a molecular (non-fibrillar) state.

Conclusions:

  • Nanoscale PLL-Fn films effectively mimic ECM structure and composition.
  • The observed fibronectin aggregation impacts film growth and charge properties.
  • These ECM-mimicking films positively influence pre-osteoblastic cell behavior, promoting proliferation and maintaining fibronectin bioactivity.