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Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
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Isolation and primary structures of elephant adrenocorticotropin and beta-lipotropin

C H Li1, M M Oosthuizen, D Chung

  • 1Laboratory of Molecular Endocrinology, University of California, San Francisco.

International Journal of Peptide and Protein Research
|December 1, 1988
PubMed

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The endoplasmic reticulum (ER) of pancreatic β-cells synthesizes preproinsulin, which consists of a signal peptide, A and B chains, and a C-peptide. Preproinsulin is then cleaved and folded into proinsulin, which translocates to the Golgi apparatus for sorting and packaging into secretory granules. In these granules, enzymatic clipping generates insulin and C-peptide.
Damage or functional impairment of β-cells inhibits insulin production, leading to diabetes. Diabetes treatment primarily uses...