Related Experiment Video
Updated: Mar 1, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Comparative analysis of cherry virus A genome sequences assembled from deep sequencing data
Prasad Kesanakurti1, Mark Belton2, Hanaa Saeed1
1Centre for Plant Health, Canadian Food Inspection Agency, 8801 East Saanich Road, North Saanich, BC, V8L 1H3, Canada.
Abstract:
Cherry virus A (CVA) is a ubiquitous graft-transmissible virus that mainly infects Prunus spp. Next-generation sequencing was applied to 39 tree fruit specimens infected with CVA, and 75 full and 16 partial-length CVA genome sequences were assembled. Phylogenetic analysis of these and 11 previously sequenced CVA genomes resulted in six major clusters with no observable relationship between the host and the assembled genome sequences. Recombination analysis detected four recombinants. Consistent single-nucleotide polymorphism (SNP) patterns were observed between the 75 full-length genomes and their sequence clouds, which supports a quasispecies model for CVA evolution.
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Genome Annotation and Assembly
Size and Structure of Viral Genomes
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Viruses with RNA Genomes
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

