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An arylboronate locked fluorescent probe for hypochlorite.

Leilei Shi1, Xin Li, Min Zhou

  • 1Department of Biomedical Engineering, College of Engineering and Applied Sciences, Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, Jiangsu 210093, China. weihui@nju.edu.cn ybding@njau.edu.cn.

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A novel fluorescent probe R1 detects hypochlorite (ClO-) selectively and sensitively. Its unique design ensures accurate measurements across a wide pH range, even in the presence of other reactive oxygen species.

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Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Biomedical Diagnostics

Background:

  • Hypochlorite (ClO-) is a key reactive oxygen species involved in various biological processes and oxidative stress.
  • Developing selective and sensitive probes for ClO- detection is crucial for understanding its role in health and disease.
  • Existing probes often suffer from interference by pH fluctuations or other reactive oxygen species.

Purpose of the Study:

  • To synthesize and characterize a novel arylboronate-based fluorescent probe (R1) for selective and sensitive ClO- detection.
  • To elucidate the reaction mechanism between R1 and ClO-.
  • To evaluate the probe's performance in complex biological samples across a wide pH range.

Main Methods:

  • Synthesis of the arylboronate-based fluorescent probe R1.
  • Spectroscopic analysis (fluorescence) to monitor the reaction with ClO-.
  • Mechanistic studies involving oxidation and chlorination pathways.
  • Interference studies with other reactive oxygen species (ROS) and pH variation tests.

Main Results:

  • The synthesized probe R1 demonstrated high selectivity and sensitivity for ClO- detection.
  • A detailed mechanistic study revealed an oxidation to chlorination pathway.
  • The arylboronate moiety effectively eliminated pH-dependent interference, enabling detection in a wide pH range (4.5–9.0).
  • R1 successfully detected ClO- at concentrations as low as 6.4 nM, outperforming other ROS species.

Conclusions:

  • The developed arylboronate-based fluorescent probe R1 offers a robust platform for selective and sensitive ClO- detection.
  • The probe's pH-insensitivity and selectivity make it suitable for biological applications.
  • This work provides a valuable tool for investigating biological processes involving hypochlorite.