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Updated: Mar 1, 2026
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
D1S80 polymorphism, including a new variant, in a population sample from Barcelona (Spain) using polymerase chain
Manel Gené1, Pasqual Moreno2, Emili Huguet1
1Laboratory of Forensic Hemogenetics, Department of Legal Medicine, Faculty of Medicine, University of Barcelona, 08028 Barcelona, Spain.
Abstract:
Allele and genotype frequencies for the D1S80 (MCT118) locus have been determined in a population sample from Barcelona (Spain) using the polymerase chain reaction (PCR) amplification and nonradioactive detection. In a total of 216 unrelated individuals, 24 alleles (23 common and 1 rare variant) and 67 genotypes (64 common and 3 variants) were observed. The 216 individuals came from 162 blood samples taken for paternity studies, 16 bloodstains from forensic cases, and 38 root hairs from normal individuals. The D1S80 locus demonstrated a heterozygosity of 0.7916, and a power of discrimination of 0.9731. The distribution of genotypes is in agreement with expected values according to the Hardy-Weinberg equilibrium. Additionally, the population from Barcelona differs, significantly, from the Finnish population and also, but with lower differences, from a U.S.A. Caucasian population. © 1993 Wiley-Liss, Inc.
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