Deposition of a Sorbent into a Recession on a Solid Support To Provide a New, Mechanically Robust Solid-Phase...

Area of Science:

• Analytical Chemistry
• Bioanalytical Science
• Materials Science

Background:

• Traditional solid-phase microextraction (SPME) fibers are often too fragile for direct in vivo bioanalysis.
• Existing SPME devices typically require a sheathing needle for protection during tissue penetration, adding complexity to sample collection.

Purpose of the Study:

• To develop and evaluate a novel SPME device with an integrated protective design for improved in vivo tissue sampling.
• To demonstrate the device's capability for direct, minimally invasive sampling in various biological matrices.

Main Methods:

• A new SPME device featuring a recessed extraction phase within a solid needle body was designed.
• The device's robustness was tested by puncturing fish scales and vial septa at high velocity.
• In vivo application involved sampling polyunsaturated fatty acids in salmon and tissue analysis of wild muskellunge.

Main Results:

• The recessed SPME device demonstrated high durability, withstanding forceful penetration through robust materials.
• Successful in vivo sampling was achieved in fish tissues, including repetitive skin punctures.
• The device facilitated rapid and minimally invasive analysis of target analytes in wild aquatic species.

Conclusions:

• The recessed SPME device offers a significant advancement for in vivo bioanalysis, enhancing durability and simplifying direct tissue sampling.
• This innovation enables rapid, minimally invasive, and broadly applicable SPME techniques for diverse ecological and biomedical research.
• The development opens new avenues for efficient in situ analysis of biological samples.