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Prognostic implication of leucocyte subpopulations in diffuse large B-cell lymphoma
Xiao Han1, Jing Ruan1, Wei Zhang1
1Department of Hematology, Peking Union Medical College Hospital, Beijing, China 100730.
Oncotarget
|May 27, 2017
Summary
Peripheral blood immune cell ratios, specifically CD16-monocyte/CD16+monocyte and mature neutrophil/cytotoxic NK&T cell ratios, can predict poor prognosis in diffuse large-B-cell lymphoma patients.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Host adaptive immunity and tumor microenvironment variables may predict outcomes in non-Hodgkin's lymphoma.
- Prognostic value of peripheral blood leukocyte subpopulations in diffuse large-B-cell lymphoma (DLBCL) requires further investigation.
Purpose of the Study:
- To determine the prognostic value of peripheral blood leukocyte subpopulations in diffuse large-B-cell lymphoma patients.
- To identify immune cell markers that predict overall survival and progression-free survival in DLBCL.
Main Methods:
- Prospective analysis of 16 leukocyte subpopulations using Cytodiff flow cytometry in 45 DLBCL patients.
- Cox proportional hazards model utilized to evaluate prognostic factors for survival outcomes.
Main Results:
- DLBCL patients exhibited decreased cytotoxic and non-cytotoxic NK&T cells, increased CD16+ monocytes, CD16- monocytes, and mature neutrophils.
- A decreased CD16- monocyte/CD16+ monocyte ratio and increased mature neutrophil/cytotoxic NK&T cell ratio were associated with poor progression-free and overall survival.
- A combined model incorporating the International Prognostic Index and the mature neutrophil/cytotoxic NK&T cell ratio demonstrated improved clinical outcome discrimination.
Conclusions:
- Decreased CD16-monocyte/CD16+monocyte ratio and increased mature neutrophil/cytotoxic NK&T cell ratio are predictors of poor prognosis in DLBCL.
- These findings support the investigation of cellular immunotherapy strategies for B-cell lymphoma.
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