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Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

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Published on: June 20, 2018

CWDPRNP: a tool for cervid prion sequence analysis in program R.

William L Miller¹, W David Walter²

¹Pennsylvania Cooperative Fish and Wildlife Research Unit, Department of Ecosystem Science and Management, Intercollege Graduate Degree Program in Ecology, The Pennsylvania State University, University Park, PA 16802, USA.

Bioinformatics (Oxford, England)

|May 27, 2017

Summary

This summary is machine-generated.

Chronic wasting disease (CWD) affects cervids. A new R package, CWDPRNP, aids in analyzing prion protein gene variations and spatial patterns to understand CWD susceptibility.

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Area of Science:

Wildlife disease ecology
Population genetics
Prion biology

Background:

Chronic wasting disease (CWD) is a fatal neurological illness impacting cervids.
Infectious prion proteins cause CWD, affecting ecologically and economically significant species.
Single nucleotide polymorphisms (SNPs) in the prion protein gene influence CWD susceptibility.

Purpose of the Study:

To provide wildlife managers with tools to analyze prion protein gene variability.
To determine frequencies of disease-associated alleles and genotypes.
To delineate spatial genetic patterns related to CWD.

Main Methods:

Implementation of the CWDPRNP package in program R.
Unified framework for analyzing prion protein gene variability.
Analysis of spatial genetic structure.

Main Results:

The CWDPRNP package offers a comprehensive approach to CWD genetic analysis.
Facilitates the study of prion protein gene diversity in cervid populations.
Supports the spatial mapping of genetic patterns relevant to CWD.

Conclusions:

The CWDPRNP package is a valuable resource for CWD research and management.
Understanding prion protein gene variability is crucial for managing CWD.
Spatial genetic analysis aids in predicting and mitigating CWD spread.