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Updated: Mar 1, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
Pathways in bacterial and archaeal communities dictated by ammonium stress in a high solid anaerobic digester with
1State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China.
Abstract:
Metagenomic comparisons of microbial profiles were conducted to investigate differences between the samples from steady (Day 42), ammonium-adjusting (Day 63), and ammonium-stressed (Day 102) periods during the 110-day operation of a high solid anaerobic digester of sewage sludge. Comparing to the steady period, biogas production was slightly inhibited after ammonium adjustment, during which the microbes showed higher abundance in 6 of the total 22 ammonium-related genes. In addition, among the 19 amino-acid-related genes, 9 genes involved in amino acid generation and utilization were reduced, which partially revealed the reason of deterioration of volatile solids (VSs) degradation following ammonium stress. Furthermore, although the acetoclastic pathway was to some extent inhibited with the decrease of biogas amount and content, no enhancement of genes involved in hydrogenotrophic methanogenesis was observed, elucidating the distinct role of ammonium stress in directing bacterial community structure toward the enhanced syntrophic acetate oxidation reaction.
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