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Impact of Small Molecules on β-Catenin and E-Cadherin Expression in HPV16-positive and -negative Squamous Cell

Benedikt Kramer1, Clemens Hock2, Johannes David Schultz3

  • 1Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Mannheim, Medical Faculty Mannheim, University Heidelberg, Mannheim, Germany benedikt.kramer@umm.de.

Anticancer Research
|May 29, 2017
PubMed
Abstract

Insights

Tyrosine kinase inhibitors impact β-catenin and E-cadherin expression in head and neck squamous cell carcinoma (SCC). These findings offer insights for developing targeted therapies for SCC, regardless of human papillomavirus (HPV) status.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Head and neck squamous cell carcinoma (SCC) requires validation of molecular targets.
  • β-Catenin and E-cadherin are key in cancer progression via epithelial-mesenchymal transition.
  • Human papillomavirus (HPV) status influences SCC characteristics.

Purpose of the Study:

  • To investigate the effect of tyrosine kinase inhibitors (nilotinib, dasatinib, erlotinib, gefitinib) on β-catenin and E-cadherin expression in SCC.
  • To analyze these effects in relation to HPV status.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to analyze protein expression.
  • SCC cell lines (UMSCC 11A, UMSCC 14C, CERV196) were treated with tyrosine kinase inhibitors.
  • Experiments were conducted in vitro.

Main Results:

  • All tested agents reduced β-catenin and E-cadherin expression in HPV16-negative SCC cells.
  • Gefitinib and dasatinib increased E-cadherin expression in HPV16-positive SCC cells.
  • Significant impacts on β-catenin and E-cadherin were observed in both HPV16-positive and HPV16-negative cells.

Conclusions:

  • Tyrosine kinase inhibitors significantly alter β-catenin and E-cadherin expression in head and neck SCC cells in vitro.
  • These molecular alterations may offer novel insights for targeted SCC therapies.
  • The study highlights the potential of targeting these proteins for therapeutic strategies in head and neck SCC.
Keywords:
E-cadherinHPV16dasatinibdrug resistanceerlotinibgefitinibhead and neck squamous cell carcinomanilotinibβ-Catenin

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