Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How receptor conformation depends on lipid nanodisc size: Adenosine A<sub>2A</sub> receptor and implications for class-A GPCR proteins.

Biochimica et biophysica acta. Biomembranes·2026
Same author

Large-scale endoplasmic reticulum membrane solidification spatially organises proteins under thermal or metabolic stress.

bioRxiv : the preprint server for biology·2026
Same author

Inherent Lipid Composition Abnormalities in Astrocytes Associated with Late-Onset Alzheimer's Disease (LOAD).

Cells·2026
Same author

Structural and functional modifications of neuronal lipid rafts: implications for HIV-associated neurological disorders.

Journal of neuroinflammation·2026
Same author

Apical and basolateral plasma membranes in epithelial cells have distinct lipidomes and biophysical properties.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Ether lipids influence cancer cell fate by modulating iron uptake.

Nature communications·2026

Related Experiment Video

Updated: Mar 1, 2026

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
11:41

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

Published on: June 16, 2022

2.5K

Miscibility Transition Temperature Scales with Growth Temperature in a Zebrafish Cell Line.

Margaret Burns1, Kathleen Wisser1, Jing Wu1

  • 1Department of Biophysics, University of Michigan, Ann Arbor, Michigan.

Biophysical Journal
|May 30, 2017
PubMed
Summary
This summary is machine-generated.

Zebrafish cells adjust their plasma membrane lipid composition to maintain function across different growth temperatures. This biological tuning keeps membranes near a critical miscibility transition point.

More Related Videos

Author Spotlight: Advancing Bone Cell Activity Research with Zebrafish Scales
02:30

Author Spotlight: Advancing Bone Cell Activity Research with Zebrafish Scales

Published on: January 10, 2025

6.9K
Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling
06:31

Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

Published on: May 3, 2019

7.3K

Related Experiment Videos

Last Updated: Mar 1, 2026

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
11:41

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

Published on: June 16, 2022

2.5K
Author Spotlight: Advancing Bone Cell Activity Research with Zebrafish Scales
02:30

Author Spotlight: Advancing Bone Cell Activity Research with Zebrafish Scales

Published on: January 10, 2025

6.9K
Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling
06:31

Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

Published on: May 3, 2019

7.3K

Area of Science:

  • Cell biology
  • Biophysics
  • Membrane biophysics

Background:

  • Cellular plasma membranes adapt lipid composition to environmental changes, influencing membrane function.
  • Recent studies indicate plasma membranes operate near a miscibility transition for optimal function.

Purpose of the Study:

  • Investigate how membrane transition temperature shifts with growth temperature in zebrafish (ZF4) cells.
  • Determine the timescale of membrane adaptation to temperature changes.

Main Methods:

  • Utilized giant plasma membrane vesicles (GPMVs) derived from ZF4 cells grown at various temperatures (20-32°C).
  • Analyzed GPMV lipidomes and transition temperatures.
  • Observed domain fluctuations in GPMVs.

Main Results:

  • GPMV transition temperatures consistently adjusted to ~16.7°C below growth temperature.
  • Membrane adaptation occurred over approximately 2 days upon a temperature decrease.
  • Lipidomes differed between cells grown at 28°C and 20°C.
  • Observed fluctuating domains in ZF4 GPMVs, indicative of critical membrane compositions.

Conclusions:

  • Zebrafish cells biologically tune membrane lipid composition to maintain proximity to a critical miscibility transition.
  • This adaptation ensures consistent membrane function across different growth temperatures.