Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Rapid isolation of phage lambda DNA].

E R Zabarovskiĭ, O V Turina

    Molekuliarnaia Biologiia
    |November 1, 1988
    PubMed
    Summary
    This summary is machine-generated.

    This study presents a rapid, modified protocol for isolating high-quality lambda phage DNA in 2-3 hours using standard solutions. The method yields DNA suitable for restriction enzyme digestion, cloning, and gene library construction.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Novel reference gene RPN1 for normalization of quantitative data in lung and kidney cancer].

    Molekuliarnaia biologiia·2011
    Same author

    [Two CpG-islands of SEMA3B gene: methylation in clear cell renal cell carcinoma].

    Molekuliarnaia biologiia·2010
    Same author

    [Technology of analysis of epigenetic and structural changes of epithelial tumors genome with NotI-microarrays by the example of human chromosome].

    Molekuliarnaia biologiia·2009
    Same author

    [Down-regulation of RBSP3/CTDSPL, NPRL2/G21, RASSF1A, ITGA9, HYAL1 and HYAL2 genes in non-small cell lung cancer].

    Molekuliarnaia biologiia·2009
    Same author

    [Tumor suppressor gene RBSP3 in cervical carcinoma: copy number and transcriptional level].

    Molekuliarnaia biologiia·2007
    Same author

    [Identification of changes in gene loci potentially associated with cervical cancer using NotI microarrays].

    Ukrains'kyi biokhimichnyi zhurnal (1999 )·2006

    Area of Science:

    • Molecular Biology
    • Virology
    • Biochemistry

    Background:

    • Isolating high-quality lambda phage DNA is crucial for molecular biology applications.
    • Existing methods can be time-consuming or yield suboptimal DNA quality.

    Purpose of the Study:

    • To develop a modified, rapid, and efficient procedure for lambda phage DNA preparation.
    • To ensure the isolated DNA is of high quality for downstream applications.

    Main Methods:

    • A modified protocol for lambda phage DNA isolation in two versions (micro and macro).
    • Utilized standard solutions (TE, NaCl, SDS, MgCl2, EDTA, RNAse A).
    • Omitted incubation with DNAse I and proteinase K; excluded phage concentration by PEG 6000 in the microvariant.

    Main Results:

    Related Experiment Videos

    • High-quality lambda phage DNA isolated within 2-3 hours.
    • DNA yields comparable to other methods (0.5-2 µg/ml L-broth).
    • Superior DNA quality compared to other express methods, similar to CsCl centrifugation.

    Conclusions:

    • The modified procedure provides a fast and effective method for obtaining high-quality lambda phage DNA.
    • The isolated DNA is suitable for various molecular biology techniques including restriction digestion, cloning, and gene library construction.