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Selective fluorescence detection method for selenide and selenol using monochlorobimane.

Takeshi Imai¹, Tatsuo Kurihara², Nobuyoshi Esaki²

¹Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan; Hyogo Prefectural Institute of Technology, Kobe, Hyogo 654-0037, Japan.

Analytical Biochemistry

|May 30, 2017

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Researchers developed a new method to detect selenol and selenide, crucial for understanding selenium

Area of Science:

Biochemistry
Analytical Chemistry
Selenium Metabolism

Background:

Low redox potential of selenol and selenide is vital for selenoprotein catalytic activity.
Accurate quantification of selenol and selenide is essential for understanding selenium metabolism and physiological roles.
Distinguishing selenol and selenide from thiol and sulfide is challenging due to chemical similarities.

Purpose of the Study:

To establish a sensitive, selective, quantitative, and simple method for detecting selenol and selenide.
To demonstrate the practical application of the developed method in biological samples.

Main Methods:

Utilized a reaction between selenol/selenide and monochlorobimane (MCB).
Employed ethyl acetate extraction for the product syn-(methyl,methyl)bimane.

Keywords:

Bimane Selenide Selenite Selenocysteine Selenol Selenoprotein

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Applied the MCB method for analyzing selenide production and determining selenol/selenide concentrations.

Main Results:

Successfully developed a highly sensitive, selective, quantitative, and simple MCB-based detection method.
Analyzed selenide production from selenite catalyzed by human glutathione reductase.
Determined selenide and selenol concentrations in Hepa1-6 cells.

Conclusions:

The MCB method offers a novel and effective tool for the detection of selenol and selenide in biological contexts.
This method facilitates a deeper understanding of selenium metabolism and the function of selenoproteins.
Provides a valuable new analytical tool for selenium research.