Related Experiment Video
Updated: Mar 1, 2026
Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Published on: April 17, 2009
Identification of male-specific AFLP and SCAR markers in the dioecious plant Humulus scandens
Shu-Fen Li1, Lian-Jun Wang2, Chuan-Liang Deng1
1College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, PR China.
Abstract:
In this study, 17 male-specific amplified fragment length polymorphism (AFLP) markers were identified between male and female Humulus scandens plants. BLAST analysis revealed that 7 of the 17 sex-linked sequences were highly similar to retrotransposons. Two stable male-specific sequence-characterized amplified regions (SCAR) markers were developed. These AFLP and SCAR markers are novel molecular probes that can be used efficiently to identify the genetic gender of H. scandens and may provide a basis for further investigations on the evolution of sex chromosomes.
