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Updated: Mar 1, 2026

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
Published on: April 7, 2023
[Stem Cell System. The intestinal stem cell system.]
Fumiaki Ishibashi1, Ryuichi Okamoto2, Mamoru Watanabe1
1Department of Gastroenterology and Hepatology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Japan.
Abstract:
The intestinal epithelium harbors stem cells at the bottom of crypts. Intestinal stem cells(ISCs)differentiate toward two alternative lineages of secretory or absorptive cells in a unidirectional manner. Studies have revealed factors that are indispensable for maintaining proliferation and differentiation of ISCs, which are provided from the surrounding environment. Additionally, several back-up systems compensating the sudden and massive loss of the ISC pool has been reported. Recent development of the three-dimensional culture system of ISCs in vitro has greatly contributed to elucidate these functional analyses of ISCs.
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