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Properties of native calf spleen NAD+ glycohydrolase immobilized by hydrophobic interaction
1Laboratoire de Chimie Enzymatique, CNRS UA 1182, Institut de Botanique, Strasbourg, France.
Biochemistry International
|November 1, 1988
Abstract:
NAD+ glycohydrolase, an amphipathic membrane-bound enzyme, solubilized from calf spleen microsomes with detergents and purified, was immobilized by hydrophobic interactions on octyl-Sepharose. Conditions are described for optimal adsorption on the gel. The immobilized enzyme remained catalytically active (hydrolase and transglycosidase activities) and could be used to prepare nicotinic acid analogs of NAD(P)+.