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Updated: Mar 1, 2026
Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair
Published on: March 24, 2014
Characterization of rhodamine 123 low staining cells and their dynamic changes during the injured-repaired progress
Yuqi Luo1, Haitao Yu1, Wentao Ou1
1Department of General Surgery, Nansha Hospital of Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.
Objective:
To investigate the characterization of intestinal epithelial stem cells stained by Rhodamine 123 (Rho) and analyze the dynamic changes of intestinal epithelial stem cells during the injured-repaired progress induced by 5-FU.
Methods:
Mucosal cells were obtained from adult C57BL/6J mice. The Rho stained cells were sorted using FACS. The mouse model of intestinal mucosal injured-repaired was established by injecting 5-FU and sacrificed at different time post-injection, and the middle intestines were used for detecting the percentage of Rho low staining cell fraction by FACS and detecting the expression of the intestinal epithelial stem cells marker-musashi-1 (msi-1) by RT-PCR and immunohistochemistry.
Results:
The Rho stained intestinal mucosal cells were divided into three fractions: Rho low staining fraction (12.35%), Rho middle staining fraction (35.5%) and Rho strong staining fraction (50.5%). The cells in Rho low staining fraction expressed rich msi-1 and most of which were in the G0/G1 phase of cell cycle. After treatment of 5-FU, the intestinal mucous were damaged, although the number of msi-1 positive cells has a little decrease, there was no statistical difference among the mice at different time after injection (P>0.05). However, the percentage of msi-1 positive cells increased significantly after injection (P<0.01), and the percentage of msi-1 positive cells decreased gradually during the repaired procedure of the intestinal mucous. There was significant positive correction between the percentage of msi-1 positive cells and the percentage of Rho low staining cell fraction (r=0.867, p<0.01) after 5-FU injection.
Conclusions:
The Rho low staining cell fraction from intestinal mucous contained rich intestinal epithelial stem cells, and the intestinal epithelial stem cell which expressed msi-1 played a key role in repairing the damage of intestinal mucous induced by 5-FU.
