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An encoded viral micropatch for multiplex cell-based assays through localized gene delivery
Sangkwon Han1, Hyung Jong Bae, Su Deok Kim
1QuantaMatrix Inc., Seoul National University Hospital, Daehak-ro, Jongno-gu, Seoul, South Korea. skwon@snu.ac.kr.
Lab on a Chip
|May 31, 2017
Summary
We developed shape-encoded microparticles for efficient, localized gene delivery in cell-based assays. This technology enables multiplexed screening of drug targets, advancing high-throughput drug discovery.
Area of Science:
- Biotechnology
- Cell Biology
- Drug Discovery
Background:
- High-throughput screening requires efficient methods for analyzing multiple drug targets simultaneously.
- Localized gene delivery is crucial for multiplexed cellular assays and high-content imaging.
Purpose of the Study:
- To develop a novel system for localized, multiplexed gene delivery to cells.
- To demonstrate the utility of this system in high-throughput cellular assays.
Main Methods:
- Adenoviral vector-immobilized microparticles with 2D shape-encoding were created.
- These microparticles enabled localized, patch-like gene delivery to monolayer-cultured cells.
- A multiplex G protein-coupled receptor (GPCR) internalization assay was performed.
Main Results:
- The shape-encoded microparticles successfully delivered genes to specific cellular locations.
- The system demonstrated compatibility with high-throughput cellular assays and imaging.
- Multiplexed GPCR internalization was effectively measured.
Conclusions:
- This novel microparticle system facilitates efficient, localized, and multiplexed gene delivery.
- The technology is well-suited for high-throughput cell-based assays and drug discovery.
- It enables simultaneous analysis of multiple targets in a single experiment.
