Paenibacillus aquistagni sp. nov., isolated from an artificial lake accumulating industrial wastewater

Strain 11^T was isolated from water of an artificial lake accumulating industrial wastewater on the outskirts of Celje, Slovenia. Phenotypic characterisation showed strain 11^T to be a Gram-stain positive, spore forming bacterium. The 16S rRNA gene sequence identified strain 11^T as a member of the genus Paenibacillus, closely related to Paenibacillus alvei (96.2%). Genomic similarity with P. alvei 29^T was 73.1% (gANI), 70.2% (ANIb), 86.7% (ANIm) and 21.7 ± 2.3% (GGDC). The DNA G+C content of strain 11^T was determined to be 47.5%. The predominant menaquinone of strain 11^T was identified as MK-7 and the major fatty acid as anteiso-C_15:0. The peptidoglycan was found to contain meso-diaminopimelic acid. In contrast to its close relatives P. alvei DSM 29^T, Paenibacillus apiarius DSM 5581^T and Paenibacillus profundus NRIC 0885^T, strain 11^T was found to be able to ferment D-fructose, D-mannose and D-xylose. A draft genome of strain 11^T contains a cluster of genes associated with type IV pilin synthesis usually found in clostridia, and only sporadically in other Gram-positive bacteria. Genotypic, chemotaxonomic, physiological and biochemical characteristics of strain 11^T presented in this study support the creation of a novel species within the genus Paenibacillus, for which the name Paenibacillus aquistagni sp. nov. is proposed, with strain 11^T (=ZIM B1027^T =LMG 29561^T =CCM 8679^T ) as the type strain.