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Updated: Mar 1, 2026

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
Published on: July 20, 2016
Albumin-based drug delivery using cysteine 34 chemical conjugates - important considerations and requirements
Mikael B Caspersen1, Matthias Kuhlmann2, Karl Nicholls3
1Albumedix A/S, Lottenborgvej 26, 2800 Kgs. Lyngby, Denmark.
Abstract:
The long blood circulation time of albumin has been clinically utilized as a half-life extension technology for improved drug performance. The availability of one free thiol for site-selective chemical conjugation offers an alternative approach to current genetic fusion and association-based products. This special report highlights important factors for successful conjugation that allows the reader to design and evaluate next-generation albumin conjugates. Albumin type, available conjugation chemistries, linker length, animal models and influence of conjugation on albumin pharmacokinetics and drug activity are discussed.
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