Related Experiment Video

Updated: Mar 1, 2026

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
10:09

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Published on: January 7, 2019

8.8K

Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases

Qincai Dong1, Chenggong Li2, Xiuhua Qu3

  • 1Laboratory of Genetic Engineering, Beijing Institute of Biotechnology, Beijing, China (mainland).

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
|May 31, 2017
PubMed

Insights

The study identified 1,034 differentially expressed genes (DEGs) regulated by c-Abl/Arg kinases. These findings offer new insights into the biological functions of c-Abl and Arg in gene regulation.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • c-Abl/Arg kinases are implicated in gene transcription regulation.
  • The global impact of c-Abl/Arg on differential gene expression remains largely uncharacterized.

Purpose of the Study:

  • To identify differentially expressed genes (DEGs) regulated by c-Abl/Arg.
  • To investigate the cellular functions and pathways associated with these DEGs.

Main Methods:

  • RNA sequencing (RNA-Seq) was performed on wild-type and c-Abl/Arg gene-silenced MCF-7 cells.
  • Differentially expressed genes (DEGs) were identified using edgeR software and validated by qRT-PCR.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were employed.

Main Results:

  • A total of 1,034 DEGs were significantly regulated by c-Abl/Arg (399 upregulated, 635 downregulated).
  • Key affected biological processes include cellular metabolism, neurodegenerative disease pathways, cAMP signaling, angiogenesis, and cell proliferation.

Conclusions:

  • c-Abl/Arg kinases play a significant role in regulating differential gene expression.
  • These findings provide novel insights into the broader biological functions of c-Abl and Arg kinases.

More Related Videos

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

863
Oncogene Expression Analysis with Alterations in pH in a Pancreatic Ductal Cell Line06:24

Oncogene Expression Analysis with Alterations in pH in a Pancreatic Ductal Cell Line

Published on: April 11, 2025

964

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
18.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.5K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.5K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K