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X-inactive specific transcript RNA in-situ hybridization as a tool for resolving specimen contamination events

Rory Crotty1, Kshitij Arora1,2, Shilpa Prasad3

  • 1Department of Pathology, Massachusetts General Hospital, Boston, MA, USA.

Histopathology
|May 31, 2017
PubMed
Abstract

No abstract available in PubMed .

Keywords:
XISTquality assurancespecimen mix-ups

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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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