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Comparative Proteome Analysis between High Lipid-Producing Strain Mucor circinelloides WJ11 and Low Lipid-Producing
Xin Tang1, Haiqin Chen1, Zhennan Gu1
1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University , Wuxi 214122, P.R. China.
Abstract:
Mucor circinelloides is one of few oleaginous fungi that produces a useful oil rich in γ-linolenic acid, but it usually only produces <25% total lipid. Nevertheless, we isolated a new strain WJ11 that can produce up to 36% lipid of cell dry weight. In this study, we have systematically analyzed the global changes in protein levels between the high lipid-producing strain WJ11 and the low lipid-producing strain CBS 277.49 (15%, lipid/cell dry weight) at lipid accumulation phase through comparative proteome analysis. Proteome analysis demonstrated that the branched-chain amino acid and lysine metabolism, glycolytic pathway, and pentose phosphate pathway in WJ11 were up-regulated, while the activities of tricarboxylic acid cycle and branch point enzyme for synthesis of isoprenoids were retarded compared with CBS 277.49. The coordinated regulation at proteome level indicate that more acetyl-CoA and NADPH are provided for fatty acid biosynthesis in WJ11 compared with CBS 277.49.
Insights
Researchers identified a high lipid-producing fungus, Mucor circinelloides WJ11, which yields up to 36% lipid. This strain shows enhanced pathways for fatty acid biosynthesis, offering potential for increased oil production.
Area of Science:
- Microbiology
- Biochemistry
- Biotechnology
Background:
- Mucor circinelloides is an oleaginous fungus known for producing valuable oils.
- Wild-type strains typically produce less than 25% total lipid.
- A novel high-lipid producing strain, WJ11, was isolated, achieving up to 36% lipid content.
Purpose of the Study:
- To compare protein expression between high (WJ11) and low (CBS 277.49) lipid-producing strains of Mucor circinelloides.
- To elucidate the molecular mechanisms underlying enhanced lipid accumulation in strain WJ11.
- To identify key metabolic pathways involved in high lipid production.
Main Methods:
- Comparative proteome analysis was employed to study protein level changes.
- Strains WJ11 and CBS 277.49 were analyzed during the lipid accumulation phase.
- Global protein expression profiles were systematically compared between the two strains.
Main Results:
- Proteome analysis revealed up-regulation of branched-chain amino acid and lysine metabolism, glycolysis, and the pentose phosphate pathway in WJ11.
- The tricarboxylic acid cycle and isoprenoid synthesis pathways were found to be retarded in WJ11 compared to CBS 277.49.
- These coordinated proteomic changes suggest increased provision of acetyl-CoA and NADPH for fatty acid biosynthesis in WJ11.
Conclusions:
- Strain WJ11 exhibits significantly enhanced lipid production capacity compared to CBS 277.49.
- Metabolic reprogramming, particularly in amino acid and carbohydrate pathways, supports higher fatty acid biosynthesis.
- The findings provide insights into optimizing Mucor circinelloides for industrial lipid production.
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