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MicroRNA-142-5p promotes cell growth and migration in renal cell carcinoma by targeting BTG3

Lingqi Liu1, Shuchao Liu1, Qixin Duan1

  • 1Department of Urology, Renmin Hospital of Wuhan UniversityWuhan 430060, China.

American Journal of Translational Research
|June 1, 2017
PubMed
Abstract

Insights

MicroRNA-142-5p (miR-142-5p) is elevated in renal cell carcinoma (RCC), promoting tumor cell proliferation and migration. Targeting miR-142-5p may offer a new therapeutic strategy for RCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are implicated in various cancers, with dysregulated levels observed in patients.
  • miRNAs show potential as therapeutic agents in cancer treatment.

Purpose of the Study:

  • To investigate the expression of miR-142-5p in human renal cell carcinoma (RCC).
  • To explore the role of miR-142-5p in RCC tumor growth and metastasis.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for miR-142-5p expression analysis.
  • Cellular assays (MTT, colony formation, Transwell, cell cycle) to assess miR-142-5p function.
  • Luciferase reporter assays to identify and confirm miR-142-5p targets.

Main Results:

  • miR-142-5p expression was significantly upregulated in RCC tissues and cell lines.
  • Overexpression of miR-142-5p enhanced RCC cell proliferation, colony formation, and migration.
  • miR-142-5p prevented G1 phase arrest in RCC cells.
  • BTG3 was identified as a direct target of miR-142-5p, and its re-expression counteracted miR-142-5p-induced proliferation.

Conclusions:

  • miR-142-5p promotes RCC cell proliferation and migration by targeting BTG3.
  • miR-142-5p acts as an onco-miRNA in RCC progression.
  • miR-142-5p represents a potential therapeutic target for RCC treatment.
Keywords:
BTG3miR-142-5pproliferationrenal cell carcinoma

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