Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative analysis of visual assessment, centiloid quantification, and clinical follow-up in patients with suspected Alzheimer's disease with [18F]Florbetaben PET imaging.

Revista espanola de medicina nuclear e imagen molecular·2026

Same author

Plasma small extracellular vesicles: Markers and mediators of atrial scar, in patients with atrial fibrillation.

Heart rhythm·2025

Same author

Delivery of monoclonal antibodies to the brain: the impact of nanocarrier structure.

Drug delivery and translational research·2025

Same author

Defective Olfactomedin-2 connects adipocyte dysfunction to obesity.

Nature communications·2025

Same author

Renin-Angiotensin System Autoantibody Network in Parkinson's Disease Patients.

Antioxidants (Basel, Switzerland)·2025

Same author

JNK1 in SF1 neurons regulates the central action of thyroid hormones on hepatic lipid metabolism.

Molecular metabolism·2025

Same journal

Altered expression of Toll-like receptor 9 in the lung tissue of adult mice generated by in vitro embryo culture and embryo transfer.

Histochemistry and cell biology·2026

Same journal

Dynamic changes in OTULIN and progranulin levels in experimental myocardial infarction and cardiac remodeling.

Histochemistry and cell biology·2026

Same journal

Eosinophil-associated matrix remodeling in a sterile granulomatous inflammation model: a temporal histopathological analysis.

Histochemistry and cell biology·2026

Same journal

Cellular accumulation of lipofuscin in the heart: implications in health and disease.

Histochemistry and cell biology·2026

Same journal

From lipofuscin accumulation to cellular dysfunction: a focus on liver pathophysiology.

Histochemistry and cell biology·2026

Same journal

Immunohistochemical study of α-keratin, loricrin, filaggrin-like protein, and transglutaminase-1 expression in orthokeratinized and parakeratinized epithelium of the tongue of domestic goose (Anser anser f. domestica) during embryonic development.

Histochemistry and cell biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 1, 2026

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Published on: April 10, 2013

Laser capture microdissection protocol for gene expression analysis in the brain.

P Garrido-Gil^1,2, P Fernandez-Rodríguez¹, J Rodríguez-Pallares^1,2

¹Laboratory of Neuroanatomy and Experimental Neurology, Department of Morphological Sciences, CIMUS, University of Santiago de Compostela, 15782, Santiago De Compostela, Spain.

Histochemistry and Cell Biology

|June 1, 2017

Summary

This summary is machine-generated.

A new laser capture microdissection protocol optimizes RNA extraction from brain tissue. This method enables high-quality gene expression analysis in specific brain regions like the substantia nigra.

Keywords:

Brain Gene expression Laser capture microdissection RNA integrity and yield RT-PCR Substantia nigra

More Related Videos

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

Related Experiment Videos

Last Updated: Mar 1, 2026

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Published on: April 10, 2013

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Laser capture microdissection (LCM) isolates specific cells for gene expression studies.
Reproducible RNA analysis protocols are lacking for brain tissue, especially the substantia nigra.
RNA's labile nature requires stringent preservation during manipulation and extraction.

Purpose of the Study:

To develop and optimize a reproducible laser capture microdissection protocol for high-quality RNA extraction from brain tissue.
To enable gene expression analysis in specific cell populations within the substantia nigra.
To overcome challenges associated with RNA degradation during tissue processing.

Main Methods:

Optimization of tissue manipulation, LCM process, and RNA extraction steps.
Utilized 20 µm-thick rat substantia nigra tissue sections on glass slides.
Employed rapid tyrosine hydroxylase immunofluorescence and a column-based RNA extraction method for 1 mm² microdissected area.

Main Results:

Developed an optimal LCM protocol yielding high-quality RNA from nigral tissue sections.
Successfully demonstrated the expression of RNA for angiotensin type 1 and type 2 receptors.
Achieved high RNA yield and integrity crucial for downstream PCR analysis.

Conclusions:

The optimized LCM protocol provides high-quality RNA suitable for gene expression studies in mammalian brain regions.
This method is applicable to both scarce and abundant gene expression analysis under various physiological and pathological conditions.
Facilitates detailed molecular investigation of specific cell populations within complex brain structures.