Related Experiment Video
Updated: Mar 1, 2026
09:49
Routine Screening Method for Microparticles in Platelet Transfusions
Published on: January 31, 2018
16.6K
Intracellular origin and ultrastructure of platelet-derived microparticles
A A Ponomareva1,2, T A Nevzorova1, E R Mordakhanova1
1Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
Journal of Thrombosis and Haemostasis : JTH
|June 1, 2017
Summary
Platelet microparticles, crucial in hemostasis and thrombosis, vary in structure and origin based on the activating stimulus. Thrombin stimulation releases microparticles containing cellular organelles, unlike other activators.
Area of Science:
- Hematology
- Cell Biology
- Biochemistry
Background:
- Platelet-derived microparticles (PMPs) are key players in hemostasis and thrombosis.
- Their formation mechanisms and structural characteristics are not fully understood.
Purpose of the Study:
- To investigate the formation, ultrastructure, and composition of PMPs.
- To analyze PMPs from human platelets stimulated with various agents.
Main Methods:
- Flow cytometry
- Transmission electron microscopy
- Scanning electron microscopy
Main Results:
- PMP structure, size, and origin depend on the stimulus.
- Thrombin induced PMPs from plasma membrane, cytoplasm, and intracellular structures.
- Some PMPs contained organelles like mitochondria and glycogen granules.
Conclusions:
- Stimulus-dependent PMP characteristics provide a structural basis for their varied roles.
- Findings aid in understanding PMP functions in physiology and pathology.
- Results may inform the development of novel diagnostic assays.
Related Concept Videos
01:18Structure and Function of Platelets
4.1K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
4.1K
01:22Formation of the Platelet Plug
10.1K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
10.1K