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Updated: Mar 1, 2026
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
Published on: August 9, 2013
Temporal Labeling of Nascent RNA Using Photoclick Chemistry in Live Cells
Sarah Nainar, Miles Kubota, Christopher McNitt1
1Department of Chemistry, University of Georgia , 140 Cedar Street, Athens, Georgia 30602, United States.
Abstract:
We report the first cellular application of a photoclick SPAAC reagent to label azide-functionalized RNA. 350 nm irradiation of a cyclopropenone caged oxo-dibenzocyclooctyne (photo-ODIBO) biotin yields formation of the SPAAC reactive species, which rapidly forms adducts with RNA containing 2'-azidoadenosine (2'N3-A). Photo-ODIBO was found to be highly stable in the presence of thiols, conferring greater stability relative to ODIBO. Light activated photo-ODIBO enabled tagging of cellular RNA, in addition to fluorescent imaging as well as enrichment of RNA in cell subpopulations via selective irradiation.
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