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Related Experiment Video

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Reprimo tissue-specific expression pattern is conserved between zebrafish and human.

Ricardo J Figueroa1, Gonzalo Carrasco-Avino2,3, Ignacio A Wichmann2,4

  • 1Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.

Plos One
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PubMed
Summary
This summary is machine-generated.

The Reprimo (RPRM) gene family

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The Reprimo (RPRM) gene family, including RPRM, RPRML, and RPRM3, are implicated in tumor suppression and cancer biomarker potential.
  • The precise physiological roles and expression patterns of the RPRM gene family remain largely uncharacterized.
  • Zebrafish offer a valuable in vivo model for studying RPRM family gene function due to conserved evolutionary retention.

Purpose of the Study:

  • To characterize the spatiotemporal expression of RPRM (rprma/rprmb), RPRML, and RPRM3 in zebrafish.
  • To investigate the conservation of RPRM gene expression patterns between zebrafish and humans.
  • To establish zebrafish as a model system for RPRM family research.

Main Methods:

  • Phylogenetic analysis to understand RPRM family evolution.
  • Whole-mount in situ hybridization (WISH) and fluorescent in situ hybridization (FISH) for spatiotemporal expression analysis in zebrafish embryos.
  • RT-qPCR in adult zebrafish and immunohistochemistry (IHC) staining in human tissues to assess expression patterns.

Main Results:

  • Zebrafish possess an infraclass duplication of RPRM (rprma/rprmb).
  • rprm (rprma/rprmb) and rprml exhibit similar spatiotemporal expression during zebrafish development, with early expression in somites and later in the notochord, brain, vasculature, and digestive tract.
  • rprm3 displays a unique expression pattern, restricted to the central nervous system (CNS).
  • Tissue-specific expression of RPRM transcripts and protein is conserved between zebrafish and humans.

Conclusions:

  • Zebrafish rprma/rprmb, rprml, and rprm3 genes have distinct developmental expression profiles.
  • The conserved expression patterns highlight the utility of zebrafish for studying RPRM gene family physiology and pathology.
  • Zebrafish serve as a powerful model for future research into the roles of the RPRM gene family in health and disease.