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Updated: Mar 1, 2026
Author Spotlight: Leptospira DNA Detection in Water for Environmental Analysis and Disease Surveillance
Published on: June 14, 2024
A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains
Douadi Benacer1, Siti Nursheena Mohd Zain1, John W Lewis2
1Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Introduction::
This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.
Methods::
Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.
Results::
The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water.
Conclusions::
Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
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